Author(s) :

Ayushi Singh, Bhavani Balasundarasekar.

Volume/Issue :

Abstract :

Antimicrobial resistance is a growing issue globally, and this necessitates alternative methods of treatment such as Quorum Quenching (QQ). Quorum-sensing is a form of intracellular communication within microorganisms that is dependent on population density and influences certain traits of the species, such as biofilm production. Biofilm production is the primary focus of this study. Biofilm is a collection of microorganisms that aggregate into an extracellular slimy matrix that functions as a protective layer. This study investigated the antibiofilm capabilities of three novel aqueous plant extracts on Staphylococcus epidermidis: Artocarpus heterophyllus (jackfruit) seeds, Coriandrum sativum (cilantro), and Cynara cardunculus (artichoke) flesh. Minimum Inhibitory Concentration assays were conducted to establish the antibacterial capabilities of the plants, with A. heterophyllus and C. cardunculus displaying the highest percentage inhibition of 81.072% and 71.588% respectively. Sub-inhibitory concentrations of half the MIC were applied in a crystal violet biofilm assay to isolate the biofilm disruption properties. Quantitatively, optical density (OD) readings identified that A. heterophyllus seeds were the most effective aqueous extract out of the treatments tested, reducing the biofilm formation by 22.279 ± 0.834%. The findings demonstrate that A. heterophyllus seed extract reduces biofilm formation and exhibits measurable antibacterial activity against S. epidermidis. While the samples were crude extracts and limited in replication, this study supports the notion that A. heterophyllus seeds have a strong potential to act as an antibacterial agent against S. epidermidis. The findings demonstrate that A. heterophyllus seed extract reduces biofilm formation activity and exhibits measurable antibacterial effects against S. epidermidis.